For a broader understanding:
Intestinal enterococci and Escherichia coli are measured in colony-forming units (CFU) per 100 mL of water.
The standard methods to be used for analysis have been set out: ISO 7899-1 or ISO 7899-2 for analysing intestinal enterococci and ISO 9308-3 or ISO 9308-1 for analysing Escherichia Coli.
In the good and excellent quality ranges, classification is determined by evaluating, to the 95th percentile, the normal data probability density function log10 of the microbiological data. It is assumed that the results found during the four previous years follow a statistical law known as "normal log". The 95th percentile is the value to which 95% of the data (microbiological analysis findings) is lower.
In the "sufficient" quality range, classification is determined by evaluating, to the 90th percentile, the normal data probability density function log10 of the microbiological data, the 90th percentile being the value to which 95% of the data (microbiological analysis findings) is lower.
Example :
See sample calculation illustrated with graphs ( here).
the percentile value is calculated as follows :
- i) Take the log10 value of all the bacterial counts in the data sequence to be assessed (if the value found is zero, use the log10 value of the minimum detection threshold of the analytical method used).
- ii) Calculate the arithmetical average of the log10 values (µ).
- iii) Calculate the standard deviation of the log10 values (σ).
The upper 90th percentile point of the data probability density function is derived from the following equation: upper 90th percentile = antilog (µ + 1,282 σ).
Calculate the standard deviation of the log10 values (s). The upper 95th percentile point of the data probability density function is derived from the following equation: upper 95th percentile = antilog (µ + 1,65 σ).
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